Journal: eLife

Article Title: Antagonism of PP2A is an independent and conserved function of HIV-1 Vif and causes cell cycle arrest

doi: 10.7554/eLife.53036

Figure Lengend Snippet: ( A–B ) HIV-infected cells from were stained with anti-LNGFR and anti-CD4 antibodies and analysed by flow cytometry before (input) and after (purified vs flow-through) selection using AFMACS. Purified cells were used for proteomic analysis. Infected cells express the SBP-ΔLNGFR cell surface affinity tag (LNGFR+) and downregulate CD4 through the action of Nef and Vpu ( ; ; ). Representative data ( A ) from three biological replicates ( B ) are shown. Vif AYR, NL4-3 Vif with both I31A and RI127/128 year mutations.

Article Snippet: Primary human CD4+ T cells were isolated from peripheral blood by density gradient centrifugation over Lympholyte-H (Cedarlane Laboratories) and negative selection using the Dynabeads Untouched Human CD4 T Cells kit (Invitrogen) according to the manufacturer’s instructions.

Techniques: Infection, Staining, Flow Cytometry, Purification, Selection

Journal: eLife

Article Title: Antagonism of PP2A is an independent and conserved function of HIV-1 Vif and causes cell cycle arrest

doi: 10.7554/eLife.53036

Figure Lengend Snippet: ( A–B ) Primary human CD4+ T cells were activated with CD3/CD28 Dynabeads and infected with HIV-AFMACS viruses 2 days post-activation at an MOI of 0.5. After a further 48 hr, cells were stained with FITC-conjugated anti-LNGFR and BV421-conjugated anti-CD4 antibodies, then fixed in 90% methanol, stained with 7-AAD and analysed by flow cytometry. Upper and lower panels show independent experiments using cells from two different donors ( A ). Green, LNGFR+ CD4- cells (HIV+); grey, LNGFR- CD4+ cells (uninfected). For each virus, the fraction of HIV+ (LNGFR+ CD4-) cells in G2/M is shown as a ratio to the fraction of uninfected (LNGFR- CD4+) cells in G2/M. Mean values are indicated.

Article Snippet: Primary human CD4+ T cells were isolated from peripheral blood by density gradient centrifugation over Lympholyte-H (Cedarlane Laboratories) and negative selection using the Dynabeads Untouched Human CD4 T Cells kit (Invitrogen) according to the manufacturer’s instructions.

Techniques: Infection, Activation Assay, Staining, Flow Cytometry

Journal: eLife

Article Title: Antagonism of PP2A is an independent and conserved function of HIV-1 Vif and causes cell cycle arrest

doi: 10.7554/eLife.53036

Figure Lengend Snippet:

Article Snippet: Primary human CD4+ T cells were isolated from peripheral blood by density gradient centrifugation over Lympholyte-H (Cedarlane Laboratories) and negative selection using the Dynabeads Untouched Human CD4 T Cells kit (Invitrogen) according to the manufacturer’s instructions.

Techniques: cDNA Library Assay, Flow Cytometry, Western Blot, Recombinant, Construct, Clone Assay, Transfection, Sample Prep, Software

Journal: Cell Stem Cell

Article Title: Adipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration

doi: 10.1016/j.stem.2017.02.009

Figure Lengend Snippet:

Article Snippet: Rat anti-CD45 , NovusBiologicals , Cat#: NB100-77417.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, cDNA Synthesis, Sample Prep, Sequencing